On October 22, 2008, Dresden Codak concluded a long-running sequence called "Hob", which focused on the character Kimiko's discovery of a post-Singularity robot and its attempted recovery by people from a future in which Earth was destroyed in a war with the artificial intelligence that once tended the planet.
On February 25th, 2013, Aaron Diaz launched a Kickstarter campaign to raise funds for a hard cover book edition of the webcomic. Dubbed The Tomorrow Girl: Dresden Codak Volume 1, it collected the first 5 years of the webcomic plus additional art and reformatted everything to fit printed media.  The campaign reached its original goal of $30,000 in less than 24 hours and ended with a total of $534,994.
The current and longest-running major story arc is Dark Science. The arc centers on Kimiko Ross, while introducing a few new characters. The arc also utilises to great effect the artistic style of decopunk, which, though notably present in the HOB arc, defines the city of Nephilopolis.
Kimiko Ross (given name Kimiko Sarai Kusanagi, middle name later given as Serena) is Dresden Codak's main character. Her uncompromising devotion to science leads her into social and militaristic conflicts throughout the comic, but also provides much of the genesis for each plot arc. The comic "Mother" alludes to Kimiko's mother's death as the reason for her resistance towards anything that would impede the progress of science or technology. At the end of the "Hob" storyline Kim was badly wounded, losing both legs, her left arm and her left eye. Following these events she uses cybernetic replacements of her own design, which she frequently adjusts and upgrades.
Dimitri and Alina Tokamak are two of Kimiko's close friends. They are shown to have impressive superpowers and Diaz describes the two of them as "probably Nuclear-Powered", a reference to the Tokamaknuclear fusionreactor that is their namesake.
Tiny Carl Jung is a tiny version of Carl Jung, "one of the fathers of modern tiny psychology". He seems to live with Kimiko, or at least to associate with Kimiko and the Tokamak siblings closely.
Rupert and Hubert are a pair of Victorian intellectuals who have moved to a magical palace on the moon to escape the insincerity of the world's leaders. They are less frequently recurring than the staple characters of the comic, and spend their time discussing scientific issues in the surreal manner typical of the comic. They do not interact with the other characters.