Dionets Liner- plying Trece Martires City, Imus City, Dasmariñas City, Bacoor City, and General Trias in Cavite; Coastal Mall (Parañaque City) and Pasay City in Metro Manila.
Dominion Bus Lines – formerly Times Transit, but a strike put it out of business until a company bought its remains from owner Santiago Rondaris and started a new company
Don Aldrin- plying Trece Martires City, Imus City, Dasmariñas City, Bacoor City, General Mariano Alvarez, Carmona and General Trias in Cavite; Coastal Mall (Parañaque City), Pasay City and Ayala (Makati City) in Metro Manila.
Lorna Express (owned & operate by Angeles D. Bayot)
Lucena Lines – plying Lucena City, Sariaya, Candelaria and Tiaong in Quezon; San Pablo City, Alaminos, and Turbina (Calamba City) in Laguna; Alabang (Muntinlupa City), Buendia (Pasay City) and Cubao (Quezon City) in Metro Manila.
Luzon Bus Inc.
Luzon Cisco Transport, Inc.
Maria de Leon – operating out of Ilocos Norte, it is dubbed "The Fastest Woman on the Road"
Philippine Rabbit – once called "The King of the Road", but, after 50 or so years its routes and stops were reduced by a crippling strike.
Pantranco South Express Inc. –ALATCO's Name Before Philtranco, Pantranco S.E.Inc. Once a major bus company in Southern Luzon Visayas & Mindanao until it change it name into Philtranco Service Enterprises Inc.
Philtranco –The Oldest Bus Company In Asia and in the Philippines, It started in 1914, its former company name is Albert Louis Ammen Transportation Company (ALATCO)
PP Bus Lines – the Second Bus Company to Enter Davao City, and is one of the Most Accident prone Bus in Visayas & Mindanao route
Quesmart transport Inc.
Raymond Transportation Inc.
RCJ Lines – an offshoot of the successful RCJ Trucking corporation
RCJ Trans – a sister company of RCJ Lines
Richford Bus Lines – a bus company formerly operated by Bocolan Liner Inc., now operated by Saint Anthony of Padua, plying from Ternate, Cavite – Lawton (Manila).
RRCG Transport – plying Batangas - Alabang/Buendia
RSL Bus Transport Corporation
Saint Rose Transit (a sister company of Giombacion Transit and GPS Transport)
Santrans – Operated by Severino and Eleanor Santos of City of San Jose Del Monte, Bulacan
Inocencio Aniceto Transportation – also known as St. Joseph. It is one of the most dominant bus companies in the 1970s and 1980s until it went bankrupt; however, it is recovering and is now maintaining its old route.
SJT (San Jose Transit)
SMTSCI (Santa Maria Transport Service Cooperative Inc)
Solid North (Pangasinan Solid North Transit, Inc.) – Operated By JAC Liner Inc.
Yellow Bus lines Incorporated – (Oldest bus line in Mindanao) and the second largest in Mindanao with 200 units its base terminal are in Koronadal City and General Santos City. It operation years are 50 years already in 2009.
A majority of these companies operate through several roads and highways, the most common of which being EDSA, Taft Avenue, Ayala Avenue, Commonwealth Avenue, Quezon Avenue, and Alabang-Zapote Road. Many of these buses also operate along both NLEx and SLEx, along with some operating via Skyway, and the rest are via Coastal Road.
A-7 Tours and Transport
AC Trans – Owned By Alberto Carating. Operates between Malanday in Valenzuela City and Baclaran in Parañaque City via Ayala. and Malanday –NAIA via EDSA
Admiral Transport Inc.
Armi Josh (formerly Aicer Trans)
Alabang Metro Link
Arabia Boy Express – a bus company that is operated by Irene Alejandro, plying Alabang (Las Piñas), Plaza Lawton (Manila) and also, plying from Baclaran to SM Fairview via EDSA Ayala ) under Darla Andrea
Bensan Trans – owned by Tas Trans Group of Companies, plying from Alabang/Pilar(Muntinlupa/Las Piñas) – Lawton/Vito Cruz(Manila)
BOTSC(Bus Operator Transport Service Cooperative)
BOVJEN – operated By Alberto Carating, also a sister company of A.C. Trans
CEM Trans. Services Inc. – formerly part of the Joanna Jesh Group, it was named after the founder of Joanna Jesh Transport and Nicholas Albert Transport, Crisinciano E. Mahilac, which was founded in 2007.
Mangie Transport – sister company of Precious Grace Transport
Marikina Auto Line Transport Corporation (MALTC) – One of the oldest bus company in the Philippines. Plying (Montalban – Baclaran via EDSA, Ayala, Aurora Blvd.), ( San Mateo – Baclaran via EDSA, Ayala, Commonwealth Ave., Batasan Road.), and ( Navotas – Baclaran via EDSA, Ayala ).
Marlea Transport – undermanaged by Jayross Lucky Seven Tours
Metro Manila Transit Corp. (MMTC) – the first bus company owned by the Philippine government under the Marcos regime, established in 1981 during the Iran–Iraq War but the operation was crippled by the EDSA People Power Revolution in 1986, and later became bankrupt in 1995. On the defunct MMTC shattered into four bus companies – United Workers Transport Corp. (Went bankrupt in 2009), DCOMMP (Drivers, Conductors, Mechanics Multipurpose) Transport Service Cooperative, Filcomtrans (went bankrupt in 1999), and Fastrans (went bankrupt in 2007).
Metro Manila Bus Co. (MMBC) – owned by JAC Liner Inc., it plys the Baclaran–SM Fairview route. It is thought to be the successor to the original Metrobus in the late 1980s to the 1990s.
PVP Liner – Started operations in 1984 by the late Panfilo V. Pajarillo, which the bus company was named after. It was dubbed as the "Morning Star" before changing into "Rising Star" with its air–conditioned units in 1995, and it currently operates the Asturias (Lawton, Santa Cruz) to Ayala route via Taft Avenue, Buendia, and Ayala Avenue. The company also operated the FTI to Navotas Terminal route via EDSA, but it was sold to CHER Transport in 2006 and now operating under the name of NAFTI Transport.
Safeway Bus Lines Inc. – sister company of Vil 5000 Inc.
St. Martin of Tours Trailways Inc. – sister company of Pamana Transport Services Inc.
Sampaguita Auto Transport (formerly RAMT)
Sta Rita Transport
Taguig Metro Link
Tas Trans Corp. – owned by Tas Trans Group of Companies, plying from Alabang/Pilar (Muntinlupa/Las Piñas) – Lawton/Vito Cruz (Manila)
Thelman Transit – sister company of Armi Josh
Universal Guiding Star Bus Line – sister company of Everlasting Bus Co., Inc.
V-1 Bus Line
Vil 5000 Inc. (formerly known as Vilfran Liner)
Valisno Express – sister company of Gasat Express and also have a Provincial Operation*
Viofel Liner Inc. (formerly Astroliner Inc.)
Voyager Express Liner
Wega Transport Corp.–(owned and operated by Eaglestar Transit Corp. an interprovincial bus plying in provinces of Western Samar, Northern Samar and Leyte.
Funride Transport (formerly WLH Trans/WLLH Liner) – known for operating King Long XMQ6119T, Nissan Diesel EXFOH buses & Higer buses, it operates in SM Fairview to – SM Southmall (Las Piñas) via Quezon Ave., Plaza Lawton (Manila) and – SM Southmall (Las Piñas City) – Plaza Lawton (Manila)via Coastal Rd..
Worthy Transport (HM Transport Group of Buses)
Universal Guiding Star Bus Line – sister company of Everlasting Bus Company, Inc.
Yohance Express Inc. – operated by First North Luzon Transit and a sister company of Joanna Jesh Transport Corp.
Note: Asterisk (*) denotes Franchise revoked by LTFRB since September 2015.