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Litmus is a water-soluble mixture of different dyes extracted from lichens, especially Roccella tinctoria. It is often absorbed onto filter paper to produce one of the oldest forms of pH indicator, used to test materials for acidity. Blue litmus paper turns red under acidic conditions and red litmus paper turns blue under basic (i.e. alkaline) conditions, with the color change occurring over the pH range 4.5-8.3 at 25 °C. Neutral litmus paper is purple. Litmus can also be prepared as an aqueous solution that functions similarly. Under acidic conditions the solution is red, and under basic conditions the solution is blue.
The litmus mixture has the CAS number 1393-92-6 and contains 10 to 15 different dyes. Most of the chemical components of litmus are likely to be the same as those of the related mixture known as orcein, but in different proportions. In contrast with orcein, the principal constituent of litmus has average molecular weight of 3300. Acid-base indicators on litmus owe their properties to a 7-hydroxyphenoxazone chromophore. Some fractions of litmus were given specific names including erythrolitmin (or erythrolein), azolitmin, spaniolitmin, leucoorcein and leucazolitmin. Azolitmin shows nearly the same effect as litmus.
Litmus can be found in different species of lichens. The dyes would be extracted from such species as Roccella tinctoria (South America), Roccella fuciformis (Angola and Madagascar), Roccella pygmaea (Algeria), Roccella phycopsis, Lecanora tartarea (Norway, Sweden), Variolaria dealbata, Ochrolechia parella, Parmotrema tinctorum and Parmelia. Currently, the main sources are Roccella montagnei (Mozambique) and Dendrographa leucophoea (California).
The main use of litmus is to test whether a solution is acidic or basic. Wet litmus paper can also be used to test water-soluble gases; the gas dissolves in the water and the resulting solution colors the litmus paper. For instance, ammonia gas, which is alkaline, colors the red litmus paper blue.
|Litmus (pH indicator)|
|below pH 4.5||above pH 8.3|
Chemical reactions other than acid-base reaction can also cause a color-change to litmus paper. For instance, chlorine gas turns blue litmus paper white – the litmus dye is bleached, due to presence of hypochlorite ions. This reaction is irreversible and therefore the litmus is not acting as an indicator in this situation.
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