Tartrate-resistant acid phosphatase

Acid phosphatase 5, tartrate resistant
PDB rendering based on 1war.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1WAR, 2BQ8
Identifiers
Symbols	ACP5; SPENCDI; TRAP
External IDs	OMIM: 171640 MGI: 87883 HomoloGene: 37499 GeneCards: ACP5 Gene
EC number	3.1.3.2
Gene Ontology Molecular function • acid phosphatase activity • ferrous iron binding • ferric iron binding Cellular component • lysosome • cytosol • integral to membrane Biological process • vitamin metabolic process • water-soluble vitamin metabolic process • riboflavin metabolic process • small molecule metabolic process Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	54	11433
Ensembl	ENSG00000102575	ENSMUSG00000001348
UniProt	P13686	Q05117
RefSeq (mRNA)	NM_001111034	NM_001102404
RefSeq (protein)	NP_001104504	NP_001095874
Location (UCSC)	Chr 19: 11.69 – 11.69 Mb	Chr 9: 22.13 – 22.14 Mb
PubMed search	[1]	[2]
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Tartrate-resistant acid phosphatase or acid phosphatase 5, tartrate resistant (ACP5 or TRAP) is a glycosylated monomeric metalloenzyme expressed in mammals.^[1] It has a molecular weight of approximately 35kDa, a basic isoelectric point (7.6-9.5), and optimal activity in acidic conditions. TRAP is synthesized as latent proenzyme and activated by proteolytic cleavage and reduction.^[2][3] It is differentiated from other mammalian acid phosphatases by its resistance to inhibition by tartrate, molecular weight and characteristic purple colour.

The mechanism of phosphate ester hydrolysis by TRAP is through a nucleophilic attack mechanism,^[4] whereby, catalysis occurs with the binding of a phosphate-substrate to the Fe²⁺ in the active site of TRAP. This is then followed by a nucleophilic attack by a hydroxide ligand on the bound phosphorus atom, resulting in cleavage of the phosphate ester bond and production of an alcohol. The exact identity and mechanism of the hydroxide ligand is unclear, but it is thought to be either a hydroxide that bridges the metal ions within the active site or a terminal hydroxide bound to Fe³⁺, with conflicting reports for both mechanisms.

TRAP expression and cell localization [edit]

Under normal circumstances, TRAP is highly expressed by osteoclasts, activated macrophages, neurons, and by the porcine endometrium during pregnancy.^[5][6] In newborn rats, TRAP is also detectable in the spleen, thymus, liver, kidneys, skin, lung, and heart at low levels. TRAP expression is increased in certain pathological conditions. These include leukaemic reticuloendotheliosis (hairy cell leukaemia), Gaucher’s disease, HIV-induced encephalopathy, osteoclastoma and osteoporosis, and metabolic bone diseases.

In osteoclasts, TRAP is localized within the ruffled border area, the lysosomes, the Golgi cisternae and vesicles.^[3]

TRAP gene, promoter organisation and transcription [edit]

Mammalian TRAP is encoded by one gene, which is localized on chromosome 19 (19p13.2-13.3) in humans, and on chromosome 9 in mice. TRAP DNA is, as expected from protein sequencing, highly conserved throughout the class mammalia. The TRAP gene has been cloned and sequenced in porcine, rat, human, and murine species.^[7] Human, murine, and porcine TRAP genes all contain 5 exons, and have the ATG codon at the beginning of exon 2, with exon 1 being non-coding. Within the exon 1 promoter, there are three distinct “tissue-specific” promoters: 1A, 1B, and 1C.^[8] This would allow TRAP expression to be tightly controlled. Transcribed from this gene is a 1.5kb mRNA with an open reading frame (ORF) of 969-975 bp encoding a 323-325 amino acid protein. In the rat, the ORF is 981 bp in length and encodes for a 327-amino acid protein. TRAP is translated as a single polypeptide. TRAP gene transcription is regulated by the Microphthalmia-associated transcription factor.^[9][10]

Physiology [edit]

The exact physiologic role(s) of TRAP is unknown, but many functions have been attributed to this protein. In knockout studies, TRAP^-/- mice exhibit mild osteopetrosis, associated with reduced osteoclast activity. These result in thickening and shortening of the cortices, formation of club-like deformities in the distal femur, and widened epiphyseal growth plates with delayed mineralization of cartilage, all of which increase with age.^[11] In TRAP overexpressing transgenic mice, mild osteoporosis occurs along with increased osteoblast activity and bone synthesis.^[12] Proposed functions of TRAP include osteopontin /bone sialoprotein dephosphorylation, the generation of reactive oxygen species (ROS), iron transport, and as a cell growth and differentiation factor.

Protein dephosphorylation and osteoclast migration [edit]

It has been shown that osteopontin and bone sialoprotein, bone matrix phosphoproteins, are highly efficient in vitro TRAP substrates, which bind to osteoclasts when phosphorylated.^[13] Upon partial dephosphorylation, both osteopontin and bone sialoprotein are incapable of binding to osteoclasts. From this effect, it has been hypothesized that TRAP is secreted from the ruffled border, dephosphorylates osteopontin and allows osteoclast migration, and further resorption to occur.

ROS generation [edit]

Reactive oxygen species (ROS) are generated in macrophages and osteoclasts from superoxide (O₂^-.), which forms from the action of NADPH-oxidase on oxygen (O₂).^[14] They play an essential role in the function of phagocytic cells.

TRAP, containing a redox active iron, catalyzes the generation of ROS through Fenton chemistry:^[15]

O₂ → (NADPH-oxidase) O^{2- ∙} → (superoxide dismutase) H₂O₂ → (catalase) H₂O + O₂

TRAP-Fe³⁺ (purple) + O^{2- ∙}→ TRAP-Fe²⁺ (pink) + O₂

H₂O₂ + TRAP-Fe²⁺ (pink) → HO^∙ + HO⁻ + TRAP-Fe³⁺

producing hydroxyl radicals, hydrogen peroxide, and singlet oxygen. In osteoclasts, ROS are generated at the ruffled border and seem to be required for resorption and degradation to occur.

Iron transport [edit]

In the pregnant sow, uteroferrin is highly expressed in the uterine fluids.^[16] Due to the unique anatomy of the porcine uterus, and the specific, progesterone-induced expression of TRAP; it is hypothesized that uteroferrin acts as an iron transport protein.

Cell growth and differentiation factor [edit]

TRAP is associated with osteoblast migration to bone resorption sites, and, once there, TRAP is believed to initiate osteoblast differentiation, activation, and proliferation. This hypothesis was formed from the examination of the bone structure of TRAP-null mice. It was noted that, in addition to osteopetrosis, bone formation occurred in a haphazard manner, where the microarchitecture was highly irregular.^[17]

In TRAP overexpressing mice, it has been found that the affected mice are grossly obese. This has led to the hypothesis that TRAP has involvement in hyperplastic obesity.

References [edit]

External links [edit]

• tartrate-resistant acid phosphatase at the US National Library of Medicine Medical Subject Headings (MeSH)

v t e PDB gallery 1war: RECOMBINANT HUMAN PURPLE ACID PHOSPHATASE EXPRESSED IN PICHIA PASTORIS  2bq8: CRYSTAL STRUCTURE OF HUMAN PURPLE ACID PHOSPHATASE WITH AN INHIBITORY CONFORMATION OF THE REPRESSION LOOP