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A Vacutainer blood collection tube is a sterile glass tube with a colored rubber stopper creating a vacuum seal inside of the tube facilitating the draw of a predetermined volume of liquid. Most commonly used to collect blood samples in venipuncture, serum separator tubes. Vacutainer tubes may contain additives designed to stabilize and preserve the specimen prior to analytical testing. Tubes are available with a safety-engineered closure (rubber stopper), with a variety of labeling options and stopper colors as well as a range of draw volumes.
Vacutainer tubes were invented by Joseph Kleiner and Becton Dickinson in 1949. Vacutainer is a registered trademark of Becton Dickinson, which manufactures and sells the tubes today.
Vacutainer needle are double ended, with one side being encased in a thin rubber coating for safety. When the needle is screwed into the translucent plastic needle holder, the rubber needle is inside the holder, and the exposed needle will be inserted into the vein. When a Vacutainer tube is inserted into the holder, its rubber cap is punctured by the inner needle and the vacuum in the tube pulls blood through the needle and into the tube. The filled tube is then removed and another can be inserted and filled the same way. The amount of air evacuated from the tube predetermines how much blood will fill the tube before blood stops flowing.
All tubes are topped with a color-coded plastic or rubber cap. They often include additives that mix with the blood when collected, and the color of the tube's plastic cap indicates which additives the tube contains.
Blood collection tubes expire because over time the vacuum is lost and blood will not be drawn into the tube when the needle punctures the tube.
Vacutainer tubes may contain additional substances that preserve blood for processing in a medical laboratory. Using the wrong tube may make the blood sample unusable for the purposes requested by the doctor. These additives are typically a thin film coating that is applied using an ultrasonic nozzle.
The additives may include anticoagulants (EDTA, sodium citrate, heparin) or a gel with intermediate density between blood cells and blood plasma. Additionally, some tubes contain additives that preserve certain components of or substances within the blood, such as glucose. When the tube is centrifuged, blood is separated by density, with the blood cells sinking to the bottom of the tube and the plasma or serum on top. Tubes containing gel enable the tube to be tipped upside-down after centrifugation and transported without the blood cells remixing with the serum.
The "order of draw" refers to the sequence in which these tubes should be filled. The needle which pierces the tubes can carry additives from one tube into the next, and so the sequence is standardized so that any cross-contamination of additives will not affect laboratory results.
The plastic tube version, known as Vacutainer PLUS, was developed at B-D in the early 1990s by E. Vogler, D. Montgomery and G. Harper amongst others of the Surface Science Group as US patents 5344611, 5326535, 5320812, 5257633 and 5246666.
Vacutainers are widely used in phlebotomy in developed countries due to safety and ease of use. Vacutainers have the advantage of being prepared with additives, allowing easy multi-tube draws, and having a lower chance of hemolysis. In developing countries, it is still common to draw blood using a syringe or syringes.
The purple top tube became an issue in the O.J. Simpson murder case when the defense alleged that small droplets of blood found at the crime scene contained the preservative EDTA. Had this been true, it would have meant that the droplet might have been taken from the purple top tube used to collect Simpson's blood and planted at the crime scene.